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Test Code 00235 GI BACTERIAL PANEL BY PCR

Important Note

This test detects the following: 

  1. Salmonella spp.
  2. Campylobacter spp.
  3. Shigella spp/EIEC (Enteroinvasive E. coli)
  4. Shiga toxin (Shiga toxin producing E. coli)

Tests with a positive Shiga toxin will be reflex to further testing to detect E. coli O157.  Additional charges will apply.  This test is not able to distinguish between Shigella spp. and EIEC.

Infectious

PERFORMED AT

St. Charles Medical Center Bend

Specimen Requirements

Container Type: Vial containing Enteric Transport Media (ETM)

Speciment Type: Feces

Preferred Volume: > 1mL

Emergency Minimum Volume: 0.2 mL (200uL)

Collection Procedure: Collect stool sample in enteric transport medium or Cary Blair. If fresh stool is submitted, it must be transferred to a Cary Blair or ETM vial within 2 hrs of collection. If less than 1ml of fresh stool is submitted, when transferring to the ETM vial, the ratio of media to stool must be 2:1.  Discard enough media prior to placing stool in the vial to ensure this ratio is achieved.

Specimen Processing: Store and Transport refrigerated
Required Patient Info: Specimen source
Stability:

Fresh Stool: Room Temperature for 2 hours

Cary Blair or ETM: up to 3 days Refrigerated 

Stool in the Aptima Multitest tube: 6 days at room temperature, 30 days refrigerated, up to 3 months frozen at ≤ -20°C

Frozen Stool: Unacceptable

Unacceptable Condition: Samples that have been frozen or exposed to excessive heat; specimens contaminated with water, or soap. Fresh stool not transferred to a Cary Blair or ETM vial within 2 hrs of collection. Formed stool.

 

Method

Polymerase Chain Reaction (PCR) on Panther Fusion

CPT Codes

87505

Department

Serology

Test Schedule

M-Su 06:30 - 17:00

Synonyms

Stool, PCR, GI PCR, GIPCR, Salmonella, Shigella, STEC, Shiga Toxin, E. coli, Campy, Campylobacter